RECOMBINASE AIDED AMPLIFICATION
RAA technology, short for Recombinase Aided Amplification, is a technology that utilizes recombinase, single-stranded binding protein and DNA polymerase to perform nucleic acid amplification under isothermal conditions (the optimal temperature is 39°C).
The specific principle is: Recombinase, single-stranded binding protein, and primer form polymers to scan double-stranded DNA, unwind double-stranded DNA at sequences homologous to the primers, and single-stranded binding proteins (SSBS) bind to the untwisted single strand to prevent single-stranded DNA refolding. In the presence of energy and dNTP, DNA polymerase completes the strand extension and repeats the process.
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